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ATCC
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ATCC
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ATCC
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Journal: Materials Today Bio
Article Title: Feasibility of combining JAK1 gene editing via CRISPR-CasRx with EGCG–lactoferrin nanoparticle therapy in a microneedle-based platform for atopic dermatitis
doi: 10.1016/j.mtbio.2026.102884
Figure Lengend Snippet: Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in NIH-3T3 and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).
Article Snippet:
Techniques: Plasmid Preparation, Zeta Potential Analyzer, Agarose Gel Electrophoresis, Transmission Assay, Electron Microscopy, Fluorescence, Transfection, Expressing
Journal: Cells
Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment
doi: 10.3390/cells15050429
Figure Lengend Snippet: Representative image of the subcutaneous implantation site of the NIH/3T3 group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.
Article Snippet: The
Techniques: